R:STEM - ROHTO × Regenerative Medicine

Based on the development of cutting edge life science technology
ROHTO is challenging the "NEW" of regenerative medicine field

2013	Made full-scale inroads into regenerative medicine field
2016	Obtained the accreditation to manufacture cell products in Stem Cell Processing Center (Kizu, Kyoto Prefecture)
2017	Developed an automatic hMSC cell culture system
2017	Started a phase I/II adipose-derived hMSC clinical trial on patients with liver cirrhosis
2019	Started a phase I adipose-derived hMSC clinical trial on pneumonia patients with COVID-19
2020	Obtained the accreditation to manufacture cell products in Stem Cell Processing Center Tokyo (Koto Ward, Tokyo)
2020	Started the sales of serum-free AOF medium for hMSC worldwide
2021	Started a phase II adipose-derived hMSC clinical trial on pneumonia patients with COVID-19
2021	Acquired Olympus-RMS Corporation through M&A and strengthen orthopedic surgery area

"AOF medium"

*MSC's function after culturing with R:STEM

DO NOT contain animal-origin materials	Reduction of contamination risks from serum
Strictly manufactured under cGMP condition	Easy documentation for applications
Batch-to-batch consistency	High producibility in different experiments

Cell proliferation capacity

hMSCs cultured in R:STEM Medium show high capacity of cell proliferation and achieve efficient cell expansion.

Adipose-derived hMSCs and umbilical cord-derived hMSCs were cultured using R:STEM Medium or serum-added DMEM/F-12 (Serum Medium), and cell proliferation potential was evaluated by population doubling level (PDL) calculated over time.
Cell surface marker

Cell surface markers expression of hMSCs cultured with R:STEM Medium met the criteria proposed by International Society for Cell Therapy.

Cell surface markers expression of adipose-derived hMSCs cultured with R:STEM Medium were analyzed by flow cytometry.
Multilineage differentiation capacity

hMSCs cultured with R:STEM Medium have ability to differentiate into adipocytes, osteocytes and chondrocytes.

Adipose-derived hMSCs cultured with R:STEM Medium were induced to differentiate into either adipocytes, osteoblasts or chondrocytes. Cell differentiation was analyzed by histological staining as indicated.