AOF medium for production of exosomes/EVs

Adipose-derived and umbilical-cord derived hMSCs were cultured for 10 consecutive days using StemNavi（RS1-500） or R:STEM（EM1-500） from Passage 4 to Passage 6, and the cell proliferation capacity was evaluated based on the population doubling level (PDL) calculated over time, and cell status was observed.

(Mann-Whitney U test with Bonferroni correction, n.s. )

The amount of EVs contained in StemNavi (RS1-500) or commercial Xeno-free medium was measured using CD9/CD63 ELISA kit.

Adipose-derived and umbilical cord-derived hMSCs were cultured using StemNavi（RS1-500） or commercially available AOF media from Passage 4 to Passage 6, and the amount of EVs contained in the culture supernatant of Passage 6 was measured by CD9/CD63 ELISA kit.

(Mann-Whitney U test, *p < 0.05)

Adipose-derived and umbilical cord-derived hMSCs were cultured using StemNavi（RS1-500） or commercially available AOF media from Passage 4 to Passage 6, and the amount of HGF contained in the culture supernatant of Passage 6 was measured by CD9/CD63 ELISA kit.

(Mann-Whitney U test, *p < 0.05)