AOF medium for production of exosomes/EVs

StemNavi

Product name: hMSC expansion AOF w/o Phenol Red( Ref. No. RS1-500)
AOF medium
  • Reduction in the risk of viruses caused by the use of serum
    Not containing human/animal-derived raw materials
  • Reduction in the risk of changes in cell characteristics caused by EVs
    Medium not containing EVs
  • Reduction in cellular toxicity, including reduced cell proliferative capacity
    Not containing antibiotics
  • Reduction in the time required for lot checks
    Low variability due to the use of chemically definable ingredients
  • Reduction in the time and effort of pretreatments such as addition of supplements
    Ready-to-use medium for immediate use after thawing
Features
  • Avoidance of the risk of infection by serum-free medium that is free of raw materials of human/animal origin
  • Phenol red free
  • Small lot-to-lot difference
  • EV content in the medium is below the detection limit
  • Antibiotics free
  • Ready-to-use medium for immediate use after thawing
  • Promotes the production abilities of adipose-derived and umbilical-cord-derived Evs
  • Promotes the production abilities of HGF by adipose-derived and umbilical-cord-derived hMSCs
Product Form
  • Volume: 500mL
  • Frozen Product: Storage Temperature ≦-20℃
  • ※ This product is for research use only and cannot be used for diagnostic or clinical purposes.
Price
66,000 yen (tax included)

Investigation of cell proliferation capacity of adipose/umbilical cord-derived hMSCs

Result

StemNavi (RS1-500) causes proliferation of adipose-derived and umbilical cord-derived hMSCs

Adipose-derived hMSCs
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Umbilical cord-derived hMSCs
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Adipose-derived and umbilical-cord derived hMSCs were cultured for 10 consecutive days using StemNavi(RS1-500) or R:STEM(EM1-500) from Passage 4 to Passage 6, and the cell proliferation capacity was evaluated based on the population doubling level (PDL) calculated over time, and cell status was observed.

(Mann-Whitney U test with Bonferroni correction, n.s. )

Measurement of amount of EVs In the medium

Result

The amount of EVs in StemNavi (RS1-500) is not more than the detection limit.

Amount of EVs in the medium (pg/mL)
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The amount of EVs contained in StemNavi (RS1-500) or commercial Xeno-free medium was measured using CD9/CD63 ELISA kit.

Evaluation of EVs producing ability of adipose/umbilical cord-derived hMSCs

Result

StemNavi (RS1-500) promotes the EVs production ability of adipose-derived and umbilical cord-derived hMSCs

Adipose-derived hMSCs
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Umbilical cord-derived hMSCs
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Adipose-derived and umbilical cord-derived hMSCs were cultured using StemNavi(RS1-500) or commercially available AOF media from Passage 4 to Passage 6, and the amount of EVs contained in the culture supernatant of Passage 6 was measured by CD9/CD63 ELISA kit.

(Mann-Whitney U test, *p < 0.05)

Evaluation of HGF producing ability of adipose/umbilical cord-derived hMSCs

Result

StemNavi (RS1-500) promotes the HGF production ability of adipose-derived and umbilical cord-derived hMSCs

Adipose-derived hMSCs
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Umbilical cord-derived hMSCs
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Adipose-derived and umbilical cord-derived hMSCs were cultured using StemNavi(RS1-500) or commercially available AOF media from Passage 4 to Passage 6, and the amount of HGF contained in the culture supernatant of Passage 6 was measured by CD9/CD63 ELISA kit.

(Mann-Whitney U test, *p < 0.05)

※All the data presented was obtained by our company.