Adipose-derived hMSCs were cultured using R:STEM(EM1-500), commercially available AOF medium, or commercially available xeno-free medium from Passage 2 to Passage 4, and the cell proliferation capacity was evaluated based on the population doubling level (PDL) and population doubling time (PDT) calculated over time for cells, and the cell status was observed.
(Kruskal-Wallis test, n.s.)